Gerton Lab - Atomic force microscopy

Microscopes are mechanical devices used for viewing objects and materials so minute in size that they are undetectable by the naked eye. The process conducted with such an instrument, called Microscopy, uses the combined schools of optical science and light reflection, controlled and manipulated through lenses, to study small objects at close range.

The basic microscope consists of several complex and interrelated parts: a cylinder that provides a necessary space of air between the ocular lens (eye piece) situated at the top and the objective lens fixed at the bottom, hovering close to a stage containing an optical assembly on a rotating arm and a centered hole through which a light shines from a solid U-shaped stand beneath. Magnifying values for the ocular range through X5, X10, to X20, while the values for the objective lens has a broader span: X5, X10, X20, X40, X80, and X100. These values provide the observer with a spectrum of possible distance orientations and degrees of sharpness as are necessary for viewing and analysis.

Several different kinds of microscopes exist, each having particular features:

Optical Microscope: The first ever created. The optical microscope has one or two lenses that work to enlarge and enhance images placed between the lower-most lens and the light source.

Simple Optical Microscope—uses one lens, the convex lens, in the magnifying process. This kind of microscope was used by Anton Van Leeuwenhoek during the late-sixteen and early-seventeenth centuries, around the time that the microscope was invented.

Compound Optical Microscope—has two lenses, one for the eyepiece to serve the ocular perspective and one of short focal length for objective perspective. Multiple lenses work to minimize both chromatic and spherical aberrations so that the view is unobstructed and uncorrupted.

Stereo Microscope: This is also known as the Dissecting Microscope, and uses two separate optical shafts (for both eyes) to create a three-dimensional image of the object through two slightly different viewpoints. This kind of microscope conducts microsurgery, dissection, watch-making, small circuit board manufacturing, etc.

Inverted Microscope: This kind of microscope views objects from an inverted position than that of regular microscopes. The inverted microscope specializes in the study of cell cultures in liquid.

Petrographic Microscope: This kind of microscope features a polarizing filter, a rotating stage, and gypsum plate. Petrographic Microscopes specialize in the study of inorganic substances whose properties tend to alter through shifting perspective.

Pocket Microscope: This kind of microscope consists of a single shaft with an eye piece at one end and an adjustable objective lens at the other. This old-style microscope has a case for easy carry.

Electron Microscopes: This kind of microscope employs electron waves running parallel to a magnetic field providing higher resolution. Two Electron Microscopes are the Scanning Electron Microscope and the Transmission Electron Microscope.

Scanning Probe Microscope: This kind of microscope measures interaction between a physical probe and a sample to form a micrograph. Only surface data can be collected and analyzed from the sample. Types of Scanning Probe Microscopes include the Atomic Force Microscope, the Scanning Tunneling Microscope, the Electric Force Microscope, and the Magnetic Force Microscope.

Science wouldn’t be what it is today without the microscope, as this device is the primary instrument by which the world and all of its elements are measured and assessed. It is with the microscope that we take a look inside of ourselves so we can learn and understand who we are and how we work.

Watch the video related to atomic force microscope

Rice University professor, Dr. Hafner, explains the Atomic Force Microscope and its applications in the field of nanotechnology.

Help answer the question about atomic force microscope

How sharp do atomic force microscope tips have to be?
Smaller and sharper gives better resolution, but maybe wider and blunter gives more force?

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14 Responses to “Gerton Lab – Atomic force microscopy”

  1. WPMixer says:

    thank you for taking the time to explain some of these things

  2. aznriceboiz5 says:

    I don't know about the milipede thing but the atomic force microscope measures the atomic force against a tip of a very sharp material like tungsten. When the probe gets too close the atom or molecule presses with a force against it. If you charge something positive the tip might get closer than if the object was charged negative for example. Since they are measuring the height at which the probe comes to rest with respect to the object, they know if something is positive or negative. Thats the principle in theory

  3. Anonymous says:

    2+2=4

  4. Blogger says:

    i hope we can get deeper, i am so curious to know what reality is

  5. Free Blog says:

    yeah they was to kill us off with nano germs. look at this person and if you see him….you know what to do.

  6. Venice says:

    Optical (conventional) microscopes can magnify up to about 40 times in stereo, wide field full color…
    60 times color begins to smear (color aberration)
    This is caused by the colors not focusing at the same distance.
    Further magnification like 400X, there is no more color; the image is also very different. Difficult to explain, would need to send pictures.
    There is also the working distance, this is a microscope design criterion.
    The closer to the subject, the sharper the image, but then there is no room to work!

    Atomic microscopes can have much higher magnifications. The colors are done by software.

    There are some photos here if bugs interest you

    http://www.scharfphoto.com/

    Hope this properly answers your question

    Guru

  7. tc says:

    To the best of my knowledge, if you are dealing with purely antimatter, you would study its interactions in the exact same ways you would study matter. The physics and chemistry would be identical, except for the charge having opposite sign. They would move opposite in response to electromagnetic fields, but the difference should be only in direction.

    Yes, the main response between matter and antimatter is annihilation, but at the particle level, a collision between a proton and an antiproton could compare to electron capture of a proton. Antimatter is rather scarce and expensive to synthesize. You'd need to use your imagination to try to figure out ways you could test for something using antimatter that you couldn't test more simply and cheaply by other means. But since it's fiction, you need not be completely constrained by physical reality. You might even conjure up a property like anti-spin. Annihilation probably wouldn't gain you much over use of antimatter, so consider whether Pauli Exclusion might allow coexistence of not just a pair of opposite spin electrons, but also a quad of opposite spin and opposite anti-spin electrons. You could double the density of matter.

    Watch for the reference, due to be published in March 2009.

  8. countrthght says:

    "Air" is made of molecules, and it, therefore, follows naturally that air is matter.

    "Void molecules" seems to be a contradiction of terms.

    If you are suggesting that empty space has a "structure"…
    …that sounds like quantum space.

    Perhaps light doesn't move faster than other things.
    Perhaps light just moves "more often".

  9. XO says:

    compound light microscope

  10. Blogger says:

    It’s funny how people make the immediately jump to say this is for weapons, shows how most humans are thinking. Being able to understand the processes and structure of a cell is key to further advancements in medicine.


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